Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing bubble or particles in liquids that relates the rate of Brownian motion to particle size. A laser beam operating at 405, 488, 532 or 635 nm is passed through a prism edged glass flat within the sample chamber. The particles in suspension in the path of this laser beam scatter light in such a manner that they are easy to visualize. A CMOS camera operating at 30 frames per seconds, captures a video file of particles moving under Brownian motion within a field of view of approximately 100-micron meter x 80 micron meter x 10 micron meter.
The NanoSight NTA software analysis each particle on a frame by frame basis and then the average distance moved is calculated by the software. The equipment can measure the bubble size and the number concentration of ultrafine bubbles.
The ultrafine bubble water in the movie was created with the GaLF, we measured with NanoSight at IDEC R&D laboratories the bubble size and bubble concentrations.